УДК 574.55
Authors:
Babich O. O.,
Khizhinskaya S. V.,
Krol O. V.,
Malkov D.I.
Annotation. A popular macroalgae polysaccharide, fucoidan is a sulfated polysaccharide composed of repeating fucose units. The aim of the research was to study the antioxidant and antimicrobial activity of fucoidan from F. vesiculosus in the Baltic region. In the work, 3 types of extraction were performed — ultrasonic, aqueous at elevated temperature and extraction in a solution of 1 % hydrochloric acid. The fucose content in the extracts was determined by spectrophotometric and chromatographic methods. The amount of sulfate groups in the obtained extracts was determined by the turbidimetric method. The antioxidant activity of the extract was measured by the capture of free radicals 2-2-diphenyl-1- picrylhydrazyl (DPPH). The antimicrobial activity of the extracts was studied by the disco diffusion method. It was found that the highest yield of fucose was shown by extracts obtained at elevated temperature and with hydrochloric acid (103.80 ± 0.51 mcg / ml and 78.20 ± 0.63 mcg / ml, respectively). Polysaccharides obtained after acid treatment of algae showed inhibition of free radicals DPPH — 90.41 ± 0.41 % and high antimicrobial activity. Antimicrobial activity of aqueous extracts in relation to C. ablicans amounted to 32.0 ± 4.0 %, with respect to P. aeruginosa — 28 ± 4.0 %.
Key words: fucoidan, algae, extraction, sulfation, fucose, antioxidant and antimicrobial activity.
